Analysis of Cortisol Concentration Changes Induced by Stress in Kickboxing K1 Competition

Łukasz Rydzik1*, İsmail İlbak2, Ibrahim Ouergui3, Leonid Podrighalo4, Tomasz Pałka5

J. Sport. Res. and Innov., vol. 1, no. 1, pp. 3-10, January 2024

1Institute of Sports Sciences, University of Physical Education, 31-571 Kraków, Poland
2Institute of Health Sciences, İnönü University, 44000 Malatya, Türkiye
3High Institute of Sport and Physical Education of Kef, University of Jendouba, Jendouba 8189, Tunisia
4Department of Physical Culture and Sports Rehabilitation, Kharkiv State Academy of PhysicalCulture; Kharkiv, Ukraine
5Department of Physiology and Biochemistry, Faculty of Physical Education and Sport, University of Physical Education, 31-571 Kraków, Poland


Background: Kickboxing is a sport that imposes significant physical and psychological stress on athletes, often pushing them to the limits of their physical endurance and mental resilience. This study focuses on analyzing changes in cortisol concentration, a key stress hormone synthesized in response to stress caused by physical and mental effort during kickboxing competitions in the K1 format. Methods: The study was conducted on 20 kickboxing athletes following K1 rules, in accordance with the Helsinki Declaration and the approval of the ethical committee. Cortisol concentration levels were measured before and after fights, and statistical analyses were performed using PQ Stat software to compare pre- and post-fight levels. Results: The results indicate a significant increase in cortisol concentration levels after the fight, suggesting a strong impact of kickboxing training on cortisol levels. This may indicate the body’s natural response to intense physical effort, highlighting the role of cortisol in energy mobilization and adaptation to increased training demands. Conclusions: Monitoring of steroid hormones – cortisol seems to be essential in coaching practice, and understanding these responses allows athletes to optimize their preparations for competitions.

Keywords: Kickboxing, Cortisol, Stress Response, Physical Stress, Psychological Stress


1. Slimani M, Chaabene H, Miarka B, Franchini E, Chamari K, Cheour F. Kickboxing re-view: anthropometric, psychophysiological and activity profiles and injury epidemiology. Biol Sport. 2017 Jun;34(2):185.

2. Dosil J, editor. The Sport Psychologist’s Handbook. Wiley; 2005.

3. Akpinar Kocakulak N, Karakus M, Akkurt S, Ozdemir N, Koca F. The Effect of Dark Chocolate on Oxidative Stress Parameters After High-Intensity Kickboxing Training. Spor Bilimleri Arastirmalari Dergisi. 2023 Oct 31;8(3):473–85.

4. Obmiński Z. Comparison of The testosterone-to cortisol-ratio values obtained from hor-monal assays in saliva and serum. J Sports Med Phys Fitness. 1997;37(1):50–5.

5. Casto KV, Edwards DA. Testosterone, cortisol, and human competition. Horm Behav. 2016 Jun;82:21–37.

6. Salvador A, Suay F, González-Bono E, Serrano MA. Anticipatory cortisol, testosterone and psychological responses to judo competition in young men. Psychoneuroendocrinol-ogy. 2003 Apr;28(3):364–75.

7. Ouergui I, Davis P, Houcine N, Marzouki H, Zaouali M, Franchini E, et al. Hormonal, Physiological, and Physical Performance During Simulated Kickboxing Combat: Differ-ences Between Winners and Losers. Int J Sports Physiol Perform. 2016 May;11(4):425–31.

8. Błach W, Ouergui I, Rydzik Ł, Ambro T. Evaluation of the Body Composition and Se-lected Physiological Variables of the Skin Surface Depending on Technical and Tactical Skills of Kickboxing Athletes in K1 Style. 2021.

9. Gençoğlu C, Ulupınar S, Özbay S, Ouergui I, Franchini E. Reliability and Validity of the Kickboxing Anaerobic Speed Test. Res Q Exerc Sport. 2022 May 3;1–10.

10. Ouergui I, Hammouda O, Chtourou H, Gmada N, Franchini E. Effects of recovery type after a kickboxing match on blood lactate and performance in anaerobic tests. Asian J Sports Med. 2014 Jun;5(2):99–107.

11. Rydzik Ł, Obmiński Z, Wąsacz W, Kopańska M, Kubacki R, Bagińska M, et al. The effect of physical exercise during competitions and in simulated conditions on hormonal-neurophysiological relationships in kickboxers. Biol Sport. 2024.

12. Obmiński Z, Supiński J, Rydzik Ł, Cynarski WJ, Ozimek M, Borysiuk Z, et al. Stress Responses to One-Day Athletic Tournament in Sport Coaches: A Pilot Study. Biology (Ba-sel). 2022;11(6):1–10.

13. Pałka T, Rydzik Ł, Tota Ł, Koteja P, Ambroży T, Mucha D, et al. Concentration levels of selected hormones in judokas and the extent of their changes during a special perfor-mance test at different ambient temperatures. BMC Sports Sci Med Rehabil. 2023 Oct 23;15(1):140.

14. Kılıc Y, Cetin HN, Sumlu E, Pektas MB, Koca HB, Akar F. Effects of Boxing Matches on Metabolic, Hormonal, and Inflammatory Parameters in Male Elite Boxers. Medicina (B Aires). 2019 Jun 18;55(6):288.

15. Jarvis M. Sport Psychology: A Student’s Handbook. Routledge; 2006.

16. Zagaria A, Ando’ A, Zennaro A. Psychology: a Giant with Feet of Clay. Integr Psychol Behav Sci. 2020 Sep;54(3):521–62.

17. Jowett S, Lavallee D, editors. Social Psychology in Sport. Human Kinetics; 2007.

18. Butler RJ. Sports Psychology in Action. CRC Press; 2020.

19. Obmiński Z, Wojtkowiak M, Stupnicki R, Golec L, HAC. Effect of acceleration stress on salivary cortisol and plasma cortisol and testosterone levels in cadet pilots. J Physiol Pharmacol. 1997;48(2):193–200.

20. Paccotti P, Minetto M, Terzolo M, Ventura M, Ganzit GP, Borrione P, et al. Effects of High-Intensity Isokinetic Exercise on Salivary Cortisol in Athletes with Different Training Schedules: Relationships to Serum Cortisol and Lactate. Int J Sports Med. 2005 Nov;26(9):747–55.

21. Yoon JR, PSC. Exercise intensity-related of β-endorfin.ACTH and cortisol. J Sport Sci. 1991;3:21–32.

22. Wirtz PH, Elsenbruch S, Emini L, Rüdisüli K, Groessbauer S, Ehlert U. Perfectionism and the Cortisol Response to Psychosocial Stress in Men. Psychosom Med. 2007 Apr;69(3):249-55.

23. World Association of Kickboxing Organizations. WAKO K1-Rules. WAKO; 2020. 4–10 p.

24. Górski J. Fizjologia wysiłku i treningu fizycznego. Wydawnictwo Lekarskie, PZWL. 2019;28, 74, 148.

25. Gonzalez-Bono E, Salvador A, Serrano MA, Ricarte J. Testosterone, Cortisol, and Mood in a Sports Team Competition. Horm Behav. 1999 Feb;35(1):55–62.

26. Vacher P, Filaire E, Mourot L, Nicolas M. Stress and recovery in sports: Effects on heart rate variability, cortisol, and subjective experience. Int J Psychophysiol. 2019 Sep;143:25–35.

27. Zorn JV, Schür RR, Boks MP, Kahn RS, Joëls M, Vinkers CH. Cortisol stress reactivity across psychiatric disorders: A systematic review and meta-analysis. Psychoneuroendo-crinology. 2017 Mar;77:25–36.

28. Jones C, Gwenin C. Cortisol level dysregulation and its prevalence—Is it nature’s alarm clock? Physiol Rep. 2021 Jan 19;8(24).

29. ISS. Research regarding sport activities influence in fighting stress at sportive and non-sportive. Scientific Bulletin of Naval Academy. 2018 Dec 15;XXI(2):146–53.

30, Tsigos C, Chrousos GP. Hypothalamic–pituitary–adrenal axis, neuroendocrine factors and stress. J Psychosom Res. 2002 Oct;53(4):865–71.

31. Dongoran MF, Muhammad Fadlih A, Riyanto P. Psychological characteristics of mar-tial sports Indonesian athletes based on categories art and fight. Enferm Clin. 2020 Jun;30:500–3.

32. Rydzik Ł. Indices of technical and tactical training during kickboxing at different levels of competition in the K1 Formula. J Kinesiol Exerc Sci. 2022;32(97):1–5.